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Magnified 100X, this image depicted a Petri dish filled with trypticase soy agar medium containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP). After having been inoculated by stabbing the surface of the BAP with a non-hemolytic group A Streptococcus pyogenes (GAS) bacteria. The BAP was incubated in a carbon dioxide enriched atmosphere at 35oC for 24 hours. The culture grew bacterial colonies along the stab in the BAP. There are no clear characteristic color changes in the region surrounding the stabbed area of the BAP in which the red blood cells in the blood agar medium have been altered to some extent. This "hemolyzed zone" indicated that these bacteria appear more like alpha- or WZ-alpha colonies in nature, which means that stabbing the BAP with non-hemolytic GAS is not helpful in the identification of the non-hemolytic variants of GAS.Created: 1977
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This photograph depicted a subsurface bacterial colony of a non-hemolytic S. pyogenes growing in a Petri dish filled with trypticase soy agar with 5% sheeps blood, (BAP). A loop of diluted non-hemolytic S. pyogenes culture was put into the melted agar (50oC) just before the blood was added to the melted agar, which was then allowed to solidify. It was then incubated at 35oC for 24 hours in a normal atmosphere. There was only a very small color change in the region surrounding the colony indicating that a narrow zone of red blood cells in the medium had been altered, which meant that these bacteria were "narrow-zone"-hemolytic in nature. Among the streptococcal species this hemolytic activity is found only with "non-hemolytic GAS".Created: 1977
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Magnified 100X, this 1977 photograph depicted a Petri dish filled with trypticase soy agar medium containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP). After having been inoculated by streaking the surface of the BAP with a non-hemolytic group A Streptococcus pyogenes (GAS) bacteria. The BAP was incubated in a carbon dioxide enriched atmosphere at 35oC for 24 hours, and grew bacterial surface colonies with no characteristic color changes surrounding each colony, or in the stabbed areas. Under examination, no red blood cells in the blood agar medium had been altered, or "hemolyzed", indicating that these bacteria were indeed non-hemolytic in nature.Infection with non-hemolytic GAS can result in a range of symptoms identical to that of typical beta-hemolytic GAS:- No illness- Mild illness (strep throat or a skin infection such as impetigo)- Severe illness (necrotizing faciitis, streptococcal toxic shock syndrome)Created: 1977
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This 1977 photograph (no magnification) depicted a Petri dish filled with trypticase soy agar medium containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP) that had been inoculated by streaking and stabbing the surface of the BAP with a non-hemolytic group A Streptococcus pyogenes (GAS) bacteria. The BAP was then incubated in a carbon dioxide enriched atmosphere at 35oC for 24 hours, and grew bacterial surface colonies with no characteristic color changes surrounding each colony, or in the stabbed areas. Under examination, no red blood cells in the blood agar medium had been altered, or "hemolyzed", indicating that these bacteria were indeed non-hemolytic in nature.Infection with non-hemolytic GAS can result in a range of symptoms identical to that of typical beta-hemolytic GAS:- No illness- Mild illness (strep throat or a skin infection such as impetigo)- Severe illness (necrotizing faciitis, streptococcal toxic shock syndrome)Created: 1977
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Magnified 100x, this 1977 photograph depicted a Petri dish filled with trypticase soy agar medium containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP). After having been inoculated with Group A Streptococcus pyogenes (GAS) bacteria using a wire loop stab technique, the BAP was incubated in a carbon dioxide enriched atmosphere at 35oC for 24 hours. The culture grew bacterial colonies along the edge of the stab, a number of which were seen here. The characteristic color changes, i.e., a colorless region around the stabbed area containing colonies of GAS in which the red blood cells in the blood agar medium had been destroyed, or "hemolyzed", indicated that these bacteria were indeed beta-hemolytic in nature.Infection with GAS can result in a range of symptoms:- No illness- Mild illness (strep throat or a skin infection such as impetigo)- Severe illness (necrotizing faciitis, streptococcal toxic shock syndrome)Created: 1977
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Magnified 100x, this 1977 photograph depicted a Petri dish filled with trypticase soy agar medium containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP). A loop of diluted culture of Streptococcus pyogenes was put into the melted agar (50oC) just before the blood was added to the melted agar. The melted agar with blood was allowed to solidify, and then incubated at 35oC for 24 hours in a normal atmosphere. The culture grew subsurface bacterial colonies, one of which was seen here. The characteristic color changes, i.e., a colorless region surrounding each colony in which the red blood cells in the blood agar medium had been destroyed, or "hemolyzed", indicated that these bacteria were indeed beta-hemolytic in nature.Infection with GAS can result in a range of symptoms:- No illness- Mild illness (strep throat or a skin infection such as impetigo)- Severe illness (necrotizing faciitis, streptococcal toxic shock syndrome)Created: 1977
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Magnified 100x, this 1977 photograph depicted a Petri dish filled with trypticase soy agar medium containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP). After having been inoculated by streaking the surface of the BAP with Group A Streptococcus pyogenes (GAS) bacteria, the dish was incubated in a carbon dioxide enriched atmosphere at 35oC for 24 hours. The culture grew bacterial surface colonies. The characteristic color changes, i.e., a colorless region surrounding each colony in which the red blood cells in the blood agar medium had been destroyed, or "hemolyzed", indicated that these bacteria were indeed beta-hemolytic in nature.Infection with GAS can result in a range of symptoms:- No illness- Mild illness (strep throat or a skin infection such as impetigo)- Severe illness (necrotizing faciitis, streptococcal toxic shock syndrome)Created: 1977
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This 1977 photograph depicted a Petri dish with Streptococcus pyogenes-inoculated trypticase soy agar containing 5% defibrinated sheep's blood, i.e., blood agar plate (BAP), that had been "streaked", and "stabbed" with a wire loop, which had been dipped into primary culture medium. The BAP was incubated in a normal atmosphere at 35oC for 24 hours. In this case, the culture dish grew colonies of Gram-positive Group A beta-Streptococci (GAS) bacteria. The characteristic color changes, i.e., a clear, colorless region surrounding each colony in which the red blood cells in the blood agar medium had been destroyed, or "hemolyzed", indicated that these bacteria were indeed beta-hemolytic in nature. There is no magnification of this image.Infection with GAS can result in a range of symptoms:- No illness- Mild illness (strep throat or a skin infection such as impetigo)- Severe illness (necrotizing faciitis, streptococcal toxic shock syndrome)Created: 1977
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Hiroshi Nishimasu, F. Ann Ran, Patrick D. Hsu, Silvana Konermann, Soraya I. Shehata,
Wikimedia Commons
Description: English: the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 A ˚ resolution. Date: 1 May 2014, 11:09:15. Source: Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
https://dx.doi.org/10.1016/j.cell.2014.02.001. Author: Hiroshi Nishimasu, F. Ann Ran, Patrick D. Hsu, Silvana Konermann, Soraya I. Shehata, Naoshi Dohmae, Ryuichiro Ishitani, Feng Zhang, and Osamu Nureki.
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NIAID|sourceurl=https://flickr.com/photos/54591706@N02/52602062457%7Carchive=%7Creviewdate=2023-02-13 03:45:00|reviewlicense=cc-by-2.0|reviewer=FlickreviewR 2
Wikimedia Commons
Summary.mw-parser-output table.commons-file-information-table,.mw-parser-output.fileinfotpl-type-information{border:1px solid #a2a9b1;background-color:#f8f9fa;padding:5px;font-size:95%;border-spacing:2px;box-sizing:border-box;margin:0;width:100%}.mw-parser-output table.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{vertical-align:top}.mw-parser-output table.commons-file-information-table>tbody>tr>td,.mw-parser-output table.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:4px}.mw-parser-output.fileinfo-paramfield{background:#ccf;text-align:right;padding-right:0.4em;width:15%;font-weight:bold}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{border-top:0;padding-top:0;margin-top:-8px}@media only screen and (max-width:719px){.mw-parser-output table.commons-file-information-table,.mw-parser-output.commons-file-information-table.fileinfotpl-type-information{border-spacing:0;padding:0;word-break:break-word;width:100%!important}.mw-parser-output.commons-file-information-table>tbody,.mw-parser-output.fileinfotpl-type-information>tbody{display:block}.mw-parser-output.commons-file-information-table>tbody>tr>td,.mw-parser-output.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:0.2em 0.4em;text-align:left;text-align:start}.mw-parser-output.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{display:flex;flex-direction:column}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{margin-top:-1px}.mw-parser-output.fileinfo-paramfield{box-sizing:border-box;flex:1 0 100%;width:100%}} Description: Colorized scanning electron micrograph of Group A Streptococcus (Streptococcus pyogenes) bacteria (teal) and a human neutrophil (purple). Credit: NIAID. Date: 29 December 2022, 18:22. Source:
Streptococcus Pyogenes (Group A Strep). Author:
NIAID.
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Description: English: Beta-haemolytic colonies of Streptococcus pyogenes on 5% shhep blood agar after 48 hours of incubation. Date: 26 September 2021, 14:02:35. Source: Own work. Author:
Ajay Kumar Chaurasiya.
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Description: English: A sketch of an oropharynx with Streptococcus Pyogenes induced pharyngitis. Date: 24 January 2019, 04:35:39. Source: Own work. Author:
Sam.bulloo.
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Description: Deutsch: Streptococcus pyogenes und Enterokokken. Date: 15 April 2012. Source: Own work. Author:
Copacopac.
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NIAID|sourceurl=https://flickr.com/photos/54591706@N02/52602554746%7Carchive=%7Creviewdate=2023-02-13 03:45:03|reviewlicense=cc-by-2.0|reviewer=FlickreviewR 2
Wikimedia Commons
Summary.mw-parser-output table.commons-file-information-table,.mw-parser-output.fileinfotpl-type-information{border:1px solid #a2a9b1;background-color:#f8f9fa;padding:5px;font-size:95%;border-spacing:2px;box-sizing:border-box;margin:0;width:100%}.mw-parser-output table.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{vertical-align:top}.mw-parser-output table.commons-file-information-table>tbody>tr>td,.mw-parser-output table.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:4px}.mw-parser-output.fileinfo-paramfield{background:#ccf;text-align:right;padding-right:0.4em;width:15%;font-weight:bold}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{border-top:0;padding-top:0;margin-top:-8px}@media only screen and (max-width:719px){.mw-parser-output table.commons-file-information-table,.mw-parser-output.commons-file-information-table.fileinfotpl-type-information{border-spacing:0;padding:0;word-break:break-word;width:100%!important}.mw-parser-output.commons-file-information-table>tbody,.mw-parser-output.fileinfotpl-type-information>tbody{display:block}.mw-parser-output.commons-file-information-table>tbody>tr>td,.mw-parser-output.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:0.2em 0.4em;text-align:left;text-align:start}.mw-parser-output.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{display:flex;flex-direction:column}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{margin-top:-1px}.mw-parser-output.fileinfo-paramfield{box-sizing:border-box;flex:1 0 100%;width:100%}} Description: Colorized scanning electron micrograph of Group A Streptococcus (Streptococcus pyogenes) bacteria (blue) and a human neutrophil (purple). Credit: NIAID. Date: 29 December 2022, 18:16. Source:
Streptococcus Pyogenes (Group A Strep). Author:
NIAID.
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Description: English: Photomicrograph of Streptococcus pyogenes bacteria, 900x Mag. A pus specimen, viewed using Pappenheim's stain. Last century, infections by S. pyogenes claimed many lives especially since the organism was the most important cause of puerperal fever and scarlet fever. Streptococci Español: La bacteria Streptococcus pyogenes raza Pappenheim Pappenheim's stain of Streptococcus pyogenes bacteria @ 900x magnification. Date: 1979. Source: : This media comes from the
Centers for Disease Control and Prevention's
Public Health Image Library (PHIL), with identification number
#2110. Note: Not all PHIL images are public domain; be sure to check copyright status and credit authors and content providers.
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