Viruses in Totiviridae are non-enveloped, double-stranded RNA viruses with icosahedral geometries, and T=2 symmetry. The virion consists of a single capsid protein and is about 40 nanometers in diameter.
The genome is composed of a linear double-stranded RNA molecule of 4.6–6.7 kilobases. It contains 2 overlapping open reading frames (ORF)—gag and pol—which respectively encode the capsid protein and the RNA-dependent RNA polymerase. Some totiviruses contain a third small potential ORF.Genus Structure Symmetry Capsid Genomic arrangement Genomic segmentation Giardiavirus Icosahedral T=2 Non-enveloped Linear Monopartite Leishmaniavirus Icosahedral T=2 Non-enveloped Linear Monopartite Totivirus Icosahedral T=2 Non-enveloped Linear Monopartite Victorivirus Icosahedral T=2 Non-enveloped Linear Trichomonasvirus Icosahedral T=2 Non-enveloped Linear
Viral replication is cytoplasmic. Replication follows the double-stranded RNA virus replication model. Double-stranded RNA virus transcription is the method of transcription. Translation takes place by -1 ribosomal frameshifting, +1 ribosomal frameshifting, viral initiation, and RNA termination-reinitiation. The virus exits the host cell by cell-to-cell movement. Giardia lamblia protozoa, leishmania protozoa, protozoan trichomonas vaginalis, and fungi serve as the natural host.Genus Host details Tissue tropism Entry details Release details Replication site Assembly site Transmission Giardiavirus Protozoa None Cell receptor endocytosis Exocytosis Cytoplasm Cytoplasm Passive diffusion Leishmaniavirus Protozoa: leishmania None Cell receptor endocytosis Cell division Cytoplasm Cytoplasm Cell division Totivirus Fungi: saccharomyces cerevisiae; fungi: smut fungi None Cytoplasmic exchange, sporogenesis; hyphal anastomosis Cytoplasmic exchange, sporogenesis; hyphal anastomosis Cytoplasm Cytoplasm Cell division; sporogenesis; cell fusion Victorivirus Fungi None Unknown Unknown Cytoplasm Cytoplasm Unknown Trichomonasvirus Protozoa Endocytosis Unknown Unknown Cytoplasm Cytoplasm Unknown