Geotrypetes seraphini is viviparous and does not depend on water for breeding (Loader et al. 2004). Female Geotrypetes seraphini can carry 3 - 4 fetuses in the oviducts. The gills of the fetus are resorbed shortly after emerging from the egg membrane. At birth, the fetus is approximately 73 - 77 mm in length, and about 32% of the total length of the mother. Adult female Geotrypetes seraphini have a high demand for nutrition; so anywhere from 24 to 44 mm of yolk can be resorbed. Fetuses have a characteristic intra-oviducal multi-rowed dentition with spoon-shaped tooth crowns, which are shed right before birth or soon after. These teeth then get replaced by recurved teeth with shallow labial cusps (Wake 1987), which are used to scrape algae from rocks and leaves (Wake 1977).
Geotrypetes seraphini has different methods for capturing prey, depending on how the prey is positioned. If Geotrypetes seraphini and the prey meet head on, the caecilian will bite repeatedly. If the prey is met laterally, Geotrypetes seraphini grabs it with its jaws, pulling the prey further into its burrow, and continues by spinning its body rapidly so that the walls of the burrow are used to damage the body further (Bennett and Wake 1974).
The snake Midon acanthias is known to prey on Geotrypetes seraphini by pursuing it a short distance into its burrow. In order to escape predation, Geotrypetes seraphini uses its burrow as a refuge and rapidly retreats deeper into it (Bennett and Wake 1974).
Geotrypetes seraphini is primarily dependent on an anaerobiosis process for energy production, and becomes fatigued within a couple of minutes of high levels of activity (Bennett and Wake 1974).