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Visualizing-fusion-of-pseudotyped-HIV-1-particles-in-real-time-by-live-cell-microscopy-1742-4690-6-84-S5

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Summary[edit] DescriptionAPI English: Movie displaying an individual fusion event indicated by color separation, corresponding to still images in Figure 5A. Env.YFP (green) and MA.mCherry (red) labeled particles were coated onto a glass coverslip, and DFJ-8 cells were allowed to settle onto the virus particles. Image acquisition with a time resolution of 0.76 frames/sec was started at the time point of cell attachment to the coverslip. The video shows a section of the movie covering 38 sec and is displayed at a speed of 10 frames per second. The particle of interest is indicated by a white circle. While the Env.YFP signal vanished within 15 sec after virus-cell contact, the label of the MA domain remained punctated during the remaining period of observation. Still images of the video are shown in Figure 5A. Date 2009 Source Koch P, Lampe M, Godinez W, Müller B, Rohr K, Kräusslich H, Lehmann M (2009). "Visualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy". Retrovirology. DOI:10.1186/1742-4690-6-84. PMID 19765276. PMC: 2762461. Author Koch P, Lampe M, Godinez W, Müller B, Rohr K, Kräusslich H, Lehmann M Permission
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