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Visualizing-fusion-of-pseudotyped-HIV-1-particles-in-real-time-by-live-cell-microscopy-1742-4690-6-84-S2

509.22782538.580x360

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Summary[edit] DescriptionAPI English: Movie illustrating the visualization of retroviral fusion indicated by the gradual appearance of a diffuse membrane stain. Corresponding to the Additional files 1 and 3 the movie S2 shows the Env.YFP signals (green channel) of the coated Env.YFP and MA.mCherry double labeled particles coated onto a glass cover slip while DFJ-8 cells attach to the cover glass. The time series was started at the time point of cell attachment to the cover glass. Images were acquired at 1 frame per minute over a period of 20 minutes. The video is played at a speed of 10 frames per second. Date 2009 Source Koch P, Lampe M, Godinez W, Müller B, Rohr K, Kräusslich H, Lehmann M (2009). "Visualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy". Retrovirology. DOI:10.1186/1742-4690-6-84. PMID 19765276. PMC: 2762461. Author Koch P, Lampe M, Godinez W, Müller B, Rohr K, Kräusslich H, Lehmann M Permission
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22782538