Polarized assembly in the context of the dynamics of cell-to-cell transmission. A COS-1 cell generating MLV labeled with Gag-YFP (red) and expressing dynamin2-CFP (green) in contact with XC target cell expressing mCAT1-CFP (green) was imaged over a period of 8 h and 28 min. A z-stack of red and green images was taken every 134 s and merged into a single file to generate Video S11. Repeated rounds of establishment of contact, contact-induced virus assembly, and virus transmission are observed. The video is played at 5 frames/s. Video S11 represents the original data source for the analysis presented in Figure 12.