dcsimg

Assembly-of-the-Murine-Leukemia-Virus-Is-Directed-towards-Sites-of-Cell–Cell-Contact-pbio.1000163.s017

509.22724964.580x360

Description:

Polarized assembly in the context of the dynamics of cell-to-cell transmission. A COS-1 cell generating MLV labeled with Gag-YFP (red) and expressing dynamin2-CFP (green) in contact with XC target cell expressing mCAT1-CFP (green) was imaged over a period of 8 h and 28 min. A z-stack of red and green images was taken every 134 s and merged into a single file to generate Video S11. Repeated rounds of establishment of contact, contact-induced virus assembly, and virus transmission are observed. The video is played at 5 frames/s. Video S11 represents the original data source for the analysis presented in Figure 12.

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cc-by-3.0
author
Jin J, Sherer N, Heidecker G, Derse D, Mothes W
author
Jin J, Sherer N, Heidecker G, Derse D, Mothes W
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22724964