dcsimg

Assembly-of-the-Murine-Leukemia-Virus-Is-Directed-towards-Sites-of-Cell–Cell-Contact-pbio.1000163.s007


Description:

Visualization of de novo assembly and release of MLV in living cells. A HEK293 cell generating MLV labeled with Gag-YFP (red) was monitored using spinning disc confocal microscopy over a period of 1 h and 12 min. A z-stack of 13 images covering 4 µm was acquired every 59 s. To generate the QuickTime video, the 13 frames were merged into a single image using Improvision Volocity and Openlab software. The last frame of the QuickTime video displays the merged image of all frames of the time-lapse video. The video is played at 10 frames/s. The four labeled particles correspond to the particles A–D presented in Figure 2A.

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license
cc-by-3.0
author
Jin J, Sherer N, Heidecker G, Derse D, Mothes W
author
Jin J, Sherer N, Heidecker G, Derse D, Mothes W
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22724924