Summary DescriptionAPI English: Interaction between L. amazonensis vacuoles and L. major amastigote PVs. (A) Live imaging of macrophages previously infected with L. amazonensis-WT for 48 h and then superinfected with L. major-DsRed2 amastigotes. Video shows a macrophage, loaded with Lysotracker (green) and hosting the two species, at phase contrast and fluorescence merged channels. Image acquisition started at 72 hours after L. major-DsRed2 amastigote addition. Time after L. major-DsRed2 amastigote addition is shown (d:h:min:sec). Barâ=â20 Âµm. (B) Live multidimensional imaging of macrophages previously infected with L. amazonensis-WT for 48 h and then superinfected with L. major-DsRed2 amastigotes. Leishmania major-DsRed2 amastigotes, sheltered by tight PV, weakly stained with Lysotracker (green), were observed at the periphery of large, Lysotracker-positive, L. amazonensis-WT PV. Multidimensional images were constructed using Imaris blend filter, and rotated in different angles. Time after L. major-DsRed2 amastigote addition is shown (d:h:min). Barâ=â5 Âµm. Date December 2010 Source Video S1 from Real F, Mortara R, Rabinovitch M (2010). "Fusion between Leishmania amazonensis and Leishmania major Parasitophorous Vacuoles: Live Imaging of Coinfected Macrophages". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0000905. PMID21151877. PMC: 2998430. Author Real F, Mortara R, Rabinovitch M Permission (Reusing this file) This file is licensed under the Creative CommonsAttribution 2.5 Generic license. You are free: to share â to copy, distribute and transmit the work to remix â to adapt the work Under the following conditions: attribution â You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue This file was published in a Public Library of Science journal. Their websitestates that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise. Provenance This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.