Joscel Enardecido

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    Joscel Enardecido commented on "Nigrospora oryzae (Berk. & Broome) Petch 1924":

    N. oryzae is a weak pathogen causing grain spot of rice, sorghum, corn and some species of grasses. This fungus is generally transmitted vertically through seeds (Kumar,,2015). Microscopic examination of this fungal isolate was not done due to its failure to produce spores. Hence, molecular characterization was performed to identify the mycelia sterilia through Internal Transcribed Spacer (ITS)-gene sequencing of ribosomal DNA (ITS1 & ITS4). Real time polymerase chain reaction and sequencing procedures as well as primer sequences were used.

    about 1 year ago