Pseudomonas mendocina is a Gram-negative environmental bacterium that can cause opportunistic nosocomial infections, such as infective endocarditis^[1] and spondylodiscitis,^[2] although cases are very rare. It has potential use in bioremediation as it is able to degrade toluene.^[3] Based on 16S rRNA analysis, P. mendocina has been placed in the P. aeruginosa group.^[4]

The genome of P. mendocina NK-01 encodes PHAMCL and AO synthesis-related genes. Two putative PHAMCL- and AObiosynthetic pathways have been identified in this strain. The PHAMCL production pathway has been very clearly described in Pseudomonas species (11). The genes encoding PHAMCL synthases (PhaC1 and PhaC2) of P. mendocina NK-01, which are located at locus MDS_0568 and locus MDS_0566 of the genome, respectively, and flank the PhaZ gene (PHAMCL depolymerase, locus MDS_0567), have been identified. The alginate production pathway in Pseudomonas species has also been clearly demonstrated (12). For P. mendocina NK-01, the key enzymes mannuronic acid polymerase (MP, encoded by alg8 at locus MDS_1081), mannuronan C-5 epimerase (AlgG, encoded by algG at locus MDS_1077), and alginate lyase (AlgL, encoded by algL at locus MDS_1075) have also been clearly confirmed. Nucleotide sequence accession number. The complete genome sequence of P. mendocina NK-01 is available in GenBank under accession number CP002620.

References

1. ^ Aragone et al. (1992 Jun). "Pseudomonas mendocina, an environmental bacterium isolated from a patient with human infective endocarditis". J Clin Microbiol 30 (6): 1583–4. PMC 265335. PMID 1624580. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=265335. 
2. ^ Chi CY, Lai CH, Fung CP, Wang JH. (2005) Pseudomonas mendocina spondylodiscitis: a case report and literature review. Scandinavian Journal of Infectious Diseases 37(11-12):950-3. PMID 16308244
3. ^ Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK. (1991) Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase. Journal of Bacteriology 173(17):5315-27. PMID 1885512
4. ^ Anzai et al. (2000, Jul). "Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence". Int J Syst Evol Microbiol 50 (4): 1563–89. doi:10.1099/00207713-50-4-1563. PMID 10939664. 

{{/*Complete Genome of Pseudomonas mendocina NK-01, Which Synthesizes Medium-Chain-Length Polyhydroxyalkanoates and Alginate Oligosaccharides*/}}

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