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Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment

Abstract:

Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.

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Wikipedia

Proteus vulgaris

Proteus vulgaris is a rod-shaped, nitrate-reducing, indole+ and catalase-positive, hydrogen sulfide-producing, Gram-negative bacterium that inhabits the intestinal tracts of humans and animals. It can be found in soil, water, and fecal matter. It is grouped with the Enterobacteriaceae and is an opportunistic pathogen of humans. It is known to cause urinary tract infections and wound infections.

The term Proteus signifies changeability of form, as personified in the Homeric poems in Proteus, "the old man of the sea", who tends the sealflocks of Poseidon and has the gift of endless transformation. The first use of the term “Proteus” in bacteriological nomenclature was made by Hauser (1885), who described under this term three types of organisms which he isolated from putrefied meat. One of the three species Hauser identified was Proteus vulgaris, so this organism has a long history in microbiology.

Over the past two decades, the genus Proteus, and in particular P. vulgaris, has undergone a number of major taxonomic revisions. In 1982, P. vulgaris was separated into three biogroups on the basis of indole production. Biogroup one was indole negative and represented a new species, P. penneri, while biogroups two and three remained together as P. vulgaris.

Lab identification[edit]

According to laboratory fermentation tests, P. vulgaris ferments glucose and amygdalin, but does not ferment mannitol or lactose. P. vulgaris also tests positive for the methyl red (mixed acid fermentation) test and is also an extremely motile organism.

When P. vulgaris is tested using the API 20E identification system[1] it produces positive results for sulfur reduction, urease production, tryptophan deaminase production, indole production, sometimes positive gelatinase activity, and saccharose fermentation, and negative results for the remainder of the tests on the testing strip.

It is referenced in the Analytical Profile Index using the nine-digit code: 047602157

The optimal growing conditions of this organism is in a facultative anaerobic environment with an average temperature of about 40°C.

The Becton/Dickinson BBL Enterotube II system for identification of members of the family Enterobacteriaceae inoculated with P. vulgaris may yield the following results:

  • Positive for glucose fermentation (with gas production)
  • Negative for lysine and ornithine
  • Positive for hydrogen sulfide production and indole production
  • Negative for adonitol and lactose
  • Negative for arabinose, sorbitol and dulcitol
  • Positive for the phenylalanine test and the Harnstoff urea test

P. vulgaris can test positive or negative for citrate. All combine for a "Biocode ID of 31407" for use in the Interpretation Guide/Computer Coding and Identification System. P. vulgaris can also test urease negative in solid media (such as in Enterotube), but will be urease positive in liquid media. The CCIS code will still identify it with a negative urease test.

Proteus infections[edit]

Etiology and epidemiology[edit]

  • Nosocomial infections
  • P. mirabilis causes 90% of Proteus infections.
  • P. vulgaris and P. penneri are easily isolated from individuals in long-term care facilities and hospitals and from patients with underlying diseases or compromised immune systems.
  • Patients with recurrent infections, those with structural abnormalities of the urinary tract, those who have had urethral instrumentation, and those whose infections were acquired in the hospital have an increased frequency of infection caused by Proteus and other organisms (e.g., Klebsiella, Enterobacter, Pseudomonas, enterococci, and staphylococci)

Clinical expression[edit]

Enterobacteriaceae (of which Proteus is a member) and Pseudomonas species are the micro-organisms most commonly responsible for Gram-negative bacteremia and sepsis.

The presence of the sepsis syndrome associated with a urinary tract infection (UTI) should raise the possibility of urinary tract obstruction. This is especially true of patients who reside in long-term care facilities, who have long-term indwelling urethral catheters, or who have a known history of urethral anatomic abnormalities.

UTI obstruction

Urease production leads to precipitation of organic and inorganic compounds, which leads to struvite stone formation. Struvite stones are composed of a combination of magnesium ammonium phosphate (struvite) and calcium carbonate-apatite. Struvite stone formation can be sustained only when ammonia production is increased and the urine pH is elevated to decrease the solubility of phosphate. Both of these requirements can occur only when urine is infected with a urease-producing organism such as Proteus. Urease metabolizes urea into ammonia and carbon dioxide: urea 2 NH3 + CO2. The ammonia/ammonium buffer pair has a pK of 9.0, resulting in the combination of highly alkaline, ammonia-rich urine.

Symptoms attributable to struvite stones are uncommon. More often, women present with UTI, flank pain, or hematuria, and are found to have a persistently alkaline urine pH (>7.0).

Treatments[edit]

Known antibiotics to which P. vulgaris is sensitive:

Ciprofloxacin
Ceftazidime
Netilmicin
Sublactam or cefoperazone
Meropenem
Piperacillin/tazobactam
Unasyn

Antibiotics should be introduced in much higher doses than "normal" when P. vulgaris has infected the sinus or respiratory tissues; for example, ciprofloxacin should be introduced at a level of at least 2000 mg per day orally in such a situation, rather than the "standard" 1000 mg per day.

References[edit]

  1. ^ "API Test Strips". Archived from the original on 7 November 2008. 

See also[edit]

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