Overview

Comprehensive Description

General Description

Introduction

 

Grosmannia clavigera is a widespread pathogen of North American pine trees and a common (although not obligate) symbiont of bark beetles (family: scolytidae) of the genus Dendroctonus. G. clavigera is known as a bluestain fungus because of the characteristic blue-gray stain that it gives to infected wood. Over the course of an infection, G. clavigera spreads throughout the wood of the host tree. If left unchecked, G. clavigera infections will eventually disrupt the transport of nutrients and water which will result in the death of the host tree. G. clavigera can also prevent the flow of sap, which drastically lowers the host tree’s resistance to infestation from bark beetles, who in turn, carry spores and help propagate the fungus.

 

General Biology

 

G. clavigera is an ascomycete and as such, is characterized by having ascii that produce and discharge a special type of spore known as an ascospore. The assimilative phase of G. clavigera is spent in the phloem and sapwood of a host pine tree is and characterized by a large, blue mycellium. The cleistothecia are small (5.5-9.0 microns in diameter) but are grouped together in circular arrangements on sapwood with a diameter of 250-640 microns. The cleistothecia are globose, with a black and leathery exterior. Spore discharge occurs through an irregular rupture in the cliestothecium wall. After discharge, the cleistothecia are concave and saucer like. Each ascus produces eight ascospores, which are unicellular and semicircular in appearance. Since the primary mode of dispersal for G. clavigera is through spore adhesion to beetle symbionts, the ascospores are very sticky. This “stickyness” might also contribute to the fungus’ ability to stop nutrient transport in host trees by “plugging up” critical transport structures.

 

As a Pathogen

 

G. clavigera is an important pathogen of North American pine trees, specifically lodgepole pine (Pinus contorta), whitebark pine (P. albicaulis), ponderosa pine (P. ponderosa), western white pine (P. monticola), and limber pine (P. flexilis). A tree infected with G. clavigera shows relatively few signs or symptoms, although it is sometimes possible to notice large, blue-gray lesions underneath bark. The only sure-fire way to accurately identify whether or not a tree has been infected by a bluestain fungus is to examine the phloem and sapwood, which is typically only possible after the tree has already died.

 

Although G. clavigera can infect a wide variety of hosts, it is especially virulent in lodgepole pine. It is particularly lethal to seedlings although it has been known to kill mature lodgepole pines in less than a year after inoculation. The mechanism by which G. clavigera kills trees is thought to be a combination of its ability to prevent both nutrient transport in the phloem and the production of sap in the sapwood. The first mechanism is straight-forward in its lethality: if the tree can’t distribute nutrients, it will die. The latter mechanism, however, is usually only fatal when the fungal infection occurs simultaneously with a bark beetle infestation.

 

Another factor which adds to Clavigera’s virulence is the fact that it seems to be adept at colonizing otherwise healthy trees. This is in stark contrast with similar, closely-related fungi which are only found in trees that are already unhealthy.

 

As a Symbiont

 

G. clavigera can participate in several symbiotic relationships that aren’t directly pathogenic. The most striking relationship that exists—the one that is responsible for the great success of __G. clavigera in North America—is between G. clavigera and the bark beetle, Dendroctonus ponderosae. Since the bark beetles are interested in colonizing and laying their eggs in the same trees that G. clavigera is, these two organisms have developed a mutualistic relationship where both organisms receive some sort of benefit. The fungus receives a benefit from the bark beetles in that its spores can be carried safely and reliably between trees by bark beetles. Also, once again, since the symbiotic relationship between G. clavigera and the bark beetles is mutualistic, there are several advantages that the fungus confers to its bark beetle symbionts. As has been mentioned earlier, G. clavigera can help to exhaust the defenses of the host trees, which makes it much easier for bark beetle infestations to take hold. In addition to this, however, it has also been shown that G. clavigera can be eaten by the bark beetles and contains essential nutrients which increases the brood size and viability of the bark beetles. In an interesting complication, it has also been shown that G. clavigera shares an additional beneficial relationship with symbiotic bacteria that live on the bark beetles. In the presence of these symbiotic bacteria, spore production was greatly stimulated in G. clavigera.

 

These relationships are important because they are apparently a contributing factor to the virulence of G. clavigera and the damage potential of D. ponderosae. When either the bark beetles or fungus colonizes a tree on its own, the host tree has a reasonable chance of survival. When G. clavigera and D. ponderosae colonize a tree at the same time, however, it is almost assured that the tree will die.

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Distribution

G. clavigera is found from Mexico to Canada along the Rocky Mountain range, although there is concern that the warming climate will cause Clavigera’s range to expand further northward into the lower altitude forests of northern Canada.

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Physical Description

Diagnostic Description

G. clavigera closely resembles several ophiosomatoid fungi and can only be reliably distinguished through genetic analysis. Despite this, there are a couple of characters that can help you, very roughly, in indentifying G. clavigera:

 

The spores of G. clavigera are semicircular whereas the spores of fungi in Ceratocystiopsis and Europhium are sickle-shaped and hat-shaped, respectively.

 

Also, since G. clavigera is more virulent than other, closely related taxa, it might be possible to distinguish an outbreak of G. clavigera by the rapidity in which infected trees die.

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Look Alikes

There are several other bluestain fungi that have associations with bark beetles but are genetically distinct. The lookalikes are generally in the genus Ophiostoma and include (but are not limited to) Ophiostoma huntii, Ophiostoma montium, and Leptographium longiclavatum.

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Ecology

Habitat

G. clavigera grows best when the temperature is around 20-25C but can survive temperatures as low as -20C. Any temperatures higher than 30C will kill G. clavigera. Because of these temperature constraints G. clavigera is typically found in sub-Alpine coniferous forests where the temperature is relatively cool all year round.

 

G. clavigera’s habitat is quickly shifting, however, due to warming climates. The past several years have seen an increase of habitat types that G. clavigera can inhabit as its range pushes higher in altitude and further north.

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Relevance to Humans and Ecosystems

Benefits

Uses

Since G. clavigera is a deadly tree pathogen, much of the attention that has been paid to it has been directed toward its eradication. In recent years, however, there has a growing trend among regional Forest Services of “making the best of a bad situation” by selling blue-stained wood at premium prices. The color that G. clavigera stains wood is generally thought to be aesthetically pleasing, and as such, wood that is harvested from fungus and beetle-killed trees are more valuable than wood from their more healthy brethren. The Colorado Forest Service (a state hit particularly hard by G. clavigera and its beetle symbiont), for instance, has a program which allows, for a premium, lumber companies to harvest trees that have been killed by the bluestain fungus on Forest Service land. This program, in turn, generates funding for several campaigns that are directed toward the control of both G. clavigera and D. ponderosae.

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Wikipedia

Blue stain fungus

The blue stain fungus, Grosmannia clavigera, is a species of sac fungus. It spreads to Lodgepole pine, Ponderosa pine, Douglas-fir, and Whitebark pine trees from the body and a special structure in the heads of Mountain Pine Beetles. The blue stain fungus has evolved a relationship with mountain pine beetles that allow them to travel from tree to tree on a special structure in the beetle’s heads and stops the tree from producing resin to pitch out or kill the beetle, encouraging the pine beetle infestation occurring all along the Rocky Mountains from Mexico up into Canada. The beetles are able to mine and lay eggs while avoiding the tree’s defenses.[1] The 33 Mb genome of this fungus was sequenced in 2009.[2]

The blue stain fungus spores germinate and produce a thread like mass (mycelium) that colonizes the phloem and sapwood. Fungal spores are usually blown away by wind but blue-stain spores are “sticky”. This process eventually blocks the nutrient conducting columns of the tree draining the trees of their nutrients eventually causing the tree to starve to death.[3] The symptoms and signs of blue stain fungus are a blue-gray discoloration of sapwood in wedge shapes of recently killed trees.[4]

Preventing the blue stain would require that unfavorable conditions for the fungus be maintained such as: keeping the wood dry, protecting the wood from mountain pine beetles, and maintaining temperatures above or below ideal growing temperatures.[5] The spread of blue stain fungus can only be controlled by protecting the trees from the mountain pine beetle.

References[edit]

  1. ^ Dolgin E (2009). "Fungus genome boosts fight to save North American forests". Nature. doi:10.1038/news.2009.928. [dead link]
  2. ^ Diguistini S, Liao NY, Platt D et al. (2009). "De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data". Genome Biol 10 (9): R94. doi:10.1186/gb-2009-10-9-r94. PMC 2768983. PMID 19747388. 
  3. ^ Ballard, R.G.; Walsh, M.A.; Cole, W.E. (1984). "The penetration and growth of blue-stain fungi in the sapwood of lodgepole pine attacked by mountain pine beetle". Canadian Journal of Botany 62 (8): 1724–1729. doi:10.1139/b84-233. 
  4. ^ Christiansen, E.; Solheim, H. (2007). "The bark beetle-associated blue-stain fungus Ophiostoma polonicum can kill various spruces and Douglas fir". Journal of Forest Pathology 20 (6–7): 436–446. 
  5. ^ Hiratsuka, Y.; Chakravarty, P.; Miao, S.; Ayer, W.A. (1994). "Potential for biological protection against blue stain in Populus tremuloides with a hyphomycetous fungus, Stachybotrys cylindrospora". Canadian journal of forest research 24 (1): 174–179. doi:10.1139/x94-023. 
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