Ambystoma jeffersonianum is a long, slender salamander, ranging from 12.1 to 21 cm in length. Long digits and a wide snout help characterize this species. Bluish flecks often are scattered along the limbs and lower sides of the body, complementing the typical dark-brown or dark gray the species usually exhibits. The ventral stomach is lighter in color, usually gray.
 
This species account was based off the information in the National Audubon Society Field Guide to North American Reptiles and Amphibians (1996) .