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Botta’s serotine bat is one of the largest Eptesicus species. This bat varies in size and coloration according to its habitat. Fur short, white on the underparts while on the upperparts dense and usually long with gray hairs at the base and buff-tipped. Hairless skin extending from the snout to the eyes. Muzzle broad. Tail tip emerges from flight membrane by about 3.5 mm. Ears short with elongated tragus. The hind margin of the ear banded with a narrow furrow.