Saguinus bicolor occurs north of the Rio Amazonas, east of the Rio Negro, in the vicinity of Manaus, the capital of the state of Amazonas, Brazil. Surveys by Ayres et al. (1980, 1982) reported that the range extended only approximately 30-45 km to the north of Manaus and to the east as far as the town of Itacoatiara, approximately 100 km from the capital. Localities beyond these points indicate only the presence of S. midas. Ayres et al. (1980, 1982) and Egler (1983) supposed that it might occur as far north as the Rio Jauapurí right bank tributary of the Rio Negro, but this seems unlikely (Subirá 1998a,b). The known range today extends from the left bank of the Rio Negro, south from the Rio Cuieiras, east, north of the Rios Negro and Amazonas, to the Rio Urubu (Subirá 1998a,b). Subirá (1998b) found Saguinus bicolor only as far as 35 km from Manaus on the BR174 highway. North of there, she found only S. midas.