The thorax is densely hairy, while the abdomen alternate black and white stripes. They have very large compound eyes, their long proboscises allow them to sip nectar from a variety of flowers and the hairy hind legs facilitate the collection and transport of pollen.
Amegilla quadrifasciata are solitary bees and do not form colonies. The females lay their eggs in nests in tunnels usually excavated by themselves in loose soils. In the cells they store pollen and nectar as food for the larvae. The larvae pupate in Autumn and emerge as adult bees in March.