Barbus trimaculatus is a benthopelagic, potamodromous species. It is found in shallow water near river outlets or close to swampy areas (Konings 1990). Barbus trimaculatus commonly occurs in a wide variety of habitats, especially where there is vegetation. It occurs in main channels of large rivers, it penetrates high into some tributary systems and may also be present in isolated floodplain pools.It feeds on insects and other small organisms, and seeds of plants. Barbus trimaculatus breeds in the summer, with shoals of ripe adults moving upstream in spate after rain. Females produce as many as 8,000 eggs (Skelton 1993).