Although a number of species in the truffle genus Tuber are harvested for human consumption, the Périgord Black Truffle (Tuber melanosporum) and the Piedmont white truffle (T. magnatum) dominate the truffle trade. Although it is now cultivated more widely (e.g., in Morocco, Australia, New Zealand, Canada, the United States, Argentina, Chile and Israel), T. melanosporum is native to calcareous soils in southern Europe, occurring in Spain, France, Italy, Greece, Bulgaria, Romania, and Turkey between 40° and 48° N (Jeandroz et al 2008). The Périgord Black Truffle is the underground fruiting body of T. melanosporum, an ascomycete fungus that forms ectomycorrhizal associations with the roots of various host plants (typically oaks [Quercus], hazels [Corylus], or other broad-leaved plants). Tuber melanosporum (and some of its relatives) apparently produce chemicals that inhibit plant growth in the soil around the truffle and its plant host. Streiblova et al. (2012) reviewed the current state of knowledge regarding the mechanisms behind this phenomenon.
Truffle prices range into the hundreds (or even thousands) of Euros per kilogram. The high demand for black truffles has led to intensive efforts at cultivation. The life cycle and ecology of this species is complex (Kües and Martin 2011 and references therein), but significant progress has been made in "truffle farming". Nevertheless, commercial production does not come close to meeting demand. Many growers hope that the recently released 125 megabase haploid genome sequence for this species will provide new opportunities to achieve a deeper understanding of the biology of this truffle that will in turn allow progress in developing commercially viable cultivation techniques.
The recent detection of the very similar Chinese Black Truffle (T. indicum) in an Italian truffle plantation has raised concerns about the potential impact of this exotic truffle on the native (and more commercially valuable) T. melanosporum (Murat et al. 2008).
(Martin et al. 2010; Kües and Martin 2011 and references therein)
- Jeandroz, S., C. Murat, Y. Wang, P. Bonfante, and F. Le Tacon. 2008. Molecular phylogeny and historical biogeography of the genus Tuber, the ‘true truffles’. Journal of Biogeography 35: 815-829.
- Kües, U. and F. Martin. 2011. On the road to understanding truffles in the underground. Fungal Genetics and Biology 48: 555-560.
- Martin, F., A. Kohler, C. Murat, et al. 2010. Périgord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis. Nature 464(7291): 1033-1038.
- Murat, C., E. Zampieri, A. Vizzini, and P. Bonfante. 2008. Is the Périgord black truffle threatened by an invasive species? We dreaded it and it has happened! New Phytologist 178: 699-702.
- Streiblová, E., H. Gryndlerová, and M. Gryndler, 2012. Truffle brûlé : an efficient fungal life strategy. FEMS Microbiology Ecology 80: 1-8.
|hymenium attachment is not applicable|
|lacks a stipe|
|ecology is mycorrhizal|
Tuber melanosporum, called the black truffle, Périgord truffle or French black truffle is a species of truffle native to Southern Europe. It is one of the most expensive edible mushrooms in the world.
Italian naturalist Carlo Vittadini described the black truffle in 1831.
The round, dark brown fruiting bodies (ascocarps) have a black-brown skin with small pyramidal cusps. They have a strong, aromatic smell and normally reach a size of up to 10 cm (3.9 in). Some may be significantly larger, such as a black truffle found 2012 in Dordogne with a mass of 1.277 kg (2.82 lb).
Their flesh is initially white, then dark. It is permeated by white veins which turn brown with age. The spores are elliptical and measure about 22–55 µm by 20–35 µm. They are dark brown and covered with large spikes.
The fruiting bodies of the black truffle exude a scent reminiscent of undergrowth, strawberries, wet earth or dried fruit with a hint of cocoa. Their taste, which fully develops after the truffles are heated, is slightly peppery and bitter. If stored at room temperature, the aromatic compounds dissipate, while storage at around the freezing point (0 °C) leads to an increased synthesis of these compounds.
The aromatic compounds developed by the fruiting bodies include 2-methyl-1-butanol, isoamyl alcohol, 2-methylbutyraldehyde and 3-methylbutyraldehyde, as well as traces of sulfur compounds. One of these, dimethyl sulfide, is what attracts truffle dogs, truffle hogs and truffle flies to the fruiting bodies. Several species of yeast, which produce part of the aromatic compounds, have been isolated from Tuber melanosporum and Tuber magnatum.
The genome of the black truffle was published in 2010. It contains 125 million base pairs. 58% of the genome consists of transposable elements, and the genome contains only 7500 identified protein-encoding genes. During symbiosis, genes involved in the decomposition of plant cell walls and lipids are induced. This indicates that black truffles decompose the cell walls of their host plants at the beginning of the symbiosis.
The black truffle is morphologically very similar to the commercially less valuable Chinese truffle (Tuber indicum). To avoid fraud or misidentifications in commerce, a RFLP genetic test has been developed to distinguish the two species. Externally, they can be distinguished by their skin, which is smoother and dark red or dark brown in the Chinese truffle. Two other similar truffle species are the summer truffle (Tuber aestivum) and the winter truffle (Tuber brumale), whose flesh is of a lighter color.
Development and phenology
Black truffles grow at a depth of 5 cm (2.0 in) to 50 cm (20 in) as ectomycorrhizae, preferably in loose calcareous soil, close to the roots of their plant symbionts. These include holm oaks, French oaks, hazel, cherry and other deciduous trees. The symbiosis of holm oak saplings and black truffles has been shown to improve photosynthesis and root growth in the plant.
Black truffles suppress the growth of plants around their symbiont, creating the impression of a burnt (brûlé) area around it. They do so by parasitizing the roots of other plants, which may lead to necrosis of the root bark and the death of the parasitized plant. Moreover, part of the scent emitted by the truffles may limit the growth of other plants through oxidative stress.
The fruiting bodies develop from April to June, and are harvested from November to March.
Boars and the larvae of the truffle fly (Suillia tuberiperda), which eat the fruiting bodies, aid in the distribution of the species by excreting the indigestible spores. Their excrement likely also serves to fertilize the spores. Black truffles are sometimes found together with winter truffles, which aid the growth of black truffles in wet soils.
Up until 2010, all truffle species were thought to be homothallic, that is, capable of sexual reproduction from a single organism. Subsequent research indicated that black truffles are heterothallic, that is, sexual reproduction requires contact between mycelium of different mating types. If a tree is surrounded by mycelium of different mating types, eventually one type becomes predominant. This is relevant for the operation of truffle plantations: To improve production, planters must ensure that neighboring trees harbor mycelium of different mating types, such as by inoculating new saplings with mycelium of a particular type.
Habitat and cultivation
The natural habitat of the black truffle includes various regions in Spain, France and Italy. These are presumably the areas where the host plants found refuge during the last Ice Age. In these areas, the search for black truffles and their cultivation is a tradition going back more than 200 years.
Black truffles are now also cultivated in Australia, New Zealand, Chile and North America. Cultivation involves the planting of, for example, hazel trees whose roots are inoculated with truffle mycelium. The first fruiting bodies can be harvested about four to ten years after planting the trees.
France accounts for some 45% of the world production of black truffles, Spain for 35% and Italy for 20%. Smaller amounts are produced in Slovenia, Croatia and the Australian states of Tasmania and Western Australia. In 2005, black truffles were found in Serbia. About 80% of the French production comes from southeast France: upper Provence (départements of Vaucluse and Alpes-de-Haute-Provence), part of Dauphiné (département of Drôme), and part of Languedoc (département of Gard). 20% of the production comes from southwest France: Quercy (département of Lot) and Périgord. The largest truffle market in France (and probably also in the world) is at Richerenches in Vaucluse. The largest truffle market in southwest France is at Lalbenque in Quercy. These markets are busiest in the month of January, when the black truffles have their highest perfume.
Production has considerably diminished during the 20th century, dropping to around 20 metric tonnes per year, with peaks at around 46 tonnes in the best years. By comparison, in 1937, France produced around 1,000 metric tonnes of black truffles.
Commerce and use
With a price of about 1,000 to 2,000 euros per kilogram, black truffles are the second most expensive truffles after white truffles, and one of the most sought after edible mushrooms in the world.
In cooking, black truffles are used to refine the taste of meat, fish, soups and risotto. Unlike white truffles, the aroma of black truffles does not diminish when they are heated, but becomes more intense.
|Wikimedia Commons has media related to Tuber melanosporum.|
- There are several common names for the species in the popular literature. For example, the Field Guide to North American Truffles (Trappe, Evans & Trappe, 2007) refers to it as the "French black"; Taming the Truffle (Hall, Brown, Zambonelli, 2007) calls it the "black Périgord truffle" (but lists it under the scientific name in the index); The Book of Fungi (Roberts & Evans, 2011) calls it the "black truffle"; and the European field guide Mushrooms (Laessoe & Lincoff, 2002) calls it simply the "Perigord truffle".
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