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Eickwort et al. (1986) describe the nesting biology of Dufourea novaeangliae. The bee is solitary and univoltine, meaning it produces only one generation of offspring per year. It digs nests in the ground, and is apparently limited in its distribution to its only known pollen source, Pontederia cordata. This plant grows along shallow shores of lakes and streams. All nests were collected from Cayuga County, NY, USA. All reproductive bees overwinter and emerge in the summer. Males emerged first, early in July, with females following in the middle of the month. Females carrying pollen (indicating nest provisioning) were seen from late July to Late August. Males patrolled flowers of Pontederia cordata or nest entrances to try to mate with females. Nests excavated in late August contained 8-11 cells, although some contained only three, suggesting that these were re-nesting attempts from females whose first nests had failed. Nests occurred both scattered and in aggregations in which entrances averaged only 12.4 cm apart. Each cell was placed at the end of a lateral burrow extending from the main burrow tunnel, and these lateral tunnels were filled with dirt after the cell was provisioned and sealed.