Under a high magnification of 8000X, this scanning electron micrograph (SEM) depicted a grouping of Gram-negative Legionella pneumophila bacteria. Please see PHIL 11092 through 11152 for additional SEMs of these organisms, specifically PHIL 11097 for a colorized version of this image.
Youll note that a number of these bacteria seem to display an elongated-rod morphology. L. pneumophila are known to most frequently exhibit this configuration when grown in broth, however, they can also elongate when plate-grown cells age, as it was in this case, especially when theyve been refrigerated. The usual L. pneumophila morphology consists of stout, fat bacilli, which is the case for the vast majority of the organisms depicted here. These bacteria originated on a 1 week-old culture plate (+/- 1 day), which had incubated a single colony, at 37oC upon a buffered charcoal yeast extract (BCYE) medium with no antibiotics.