Bachelor males roost apart from females. Males residing with female groups stay for two or more reproductive seasons. Female Noctilio leporinus bear a single young each pregnancy. This appears to form the basis of a polygynous social organization.
This species tends to have pregnancies occuring from September until January, and lactation is first seen in November and continues until April. This is a general pattern, however, and it can vary with geographical location. Reproduction corresponds to seasons of greatest food availability.
Young bats don't leave the roost for their first attempts at sustained flight until nearly adult size. At that time they are slightly less than one month of age and the parents have remained at the roost throughout. This suggests a high degree of bi-parental care which may be a characteristic of this species.