English: 1= Time-lapse of Tg(tie1:H2B-eYFP) embryo shows anteriomedial rotation of newly assembled dorsal aortae. Embryos injected with QH1-A647 and imaged on the upright microscope with the dorsal side against the EC Agar culture using the 10× objective and 2×2 binning. The time lapse records strong H2B::eYFP signal starting at Stage 7–8. All of these embryos develop normally ∼15 hours. Q715: Index T000 to T033 ∼10.5 minute time points using 2×3×5 Mosaic; then Index T034 to T91 ∼10 minute time points using 2×3×7 Mosaic.