Alice and Campell used the nuclear internal transcribed spacer (ITS) to build a phylogeny of Rubus. The dataset was comprised of 57 species from 12 subgenera.
Phylogeny of Rubus (rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences, Am. J. Bot., January 1999, vol. 86, no. 1, 81-97
Yang et al. investigated the phylogeny of about 20 species with distributions in east Asian. The authors focused on the subgenera Idaeobatus (14 species). Sequence data of the second intron of the nuclear gene (LEAFY) as well as data from a prevoius study (Yang and Pak 2006) from the nuclear internal transcribed spacer (ITS) and a the plastid intergenic spacer trnL-F was used for analysis.
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