Distribution and ecology
Physcomitrella patens is an early colonist of exposed mud and earth around the edges of pools of water. P. patens has a disjunct distribution in temperate parts of the world, with the exception of South America. The standard laboratory strain is the 'Gransden' isolate, collected by H. Whitehouse from Gransden Wood, in Cambridgeshire.
Mosses share fundamental genetic and physiological processes with vascular plants, although the two lineages diverged early in land plant evolution. A comparative study between modern representatives of the two lines can give an insight into the evolution of the mechanisms behind the complexity of modern plants. It is in this context that Physcomitrella patens is used as a model organism.
Physcomitrella patens is one of a few known multicellular organisms with highly efficient homologous recombination. meaning that an exogenous DNA sequence can be targeted to a specific genomic position (a technique called gene targeting) to create knockout mosses. This approach is called reverse genetics and it is a powerful and sensitive tool to study the function of genes and, when combined with studies in higher plants like Arabidopsis thaliana, can be used to study molecular plant evolution.
The targeted deletion or alteration of moss genes relies on the integration of a short DNA strand at a defined position in the genome of the host cell. Both ends of this DNA strand are engineered to be identical to this specific gene locus. The DNA construct is then incubated with moss protoplasts in the presence of polyethylene glycol (PEG). As mosses are haploid organisms, the regenerating moss filaments (protonemata) can be directly assayed for gene targeting within 6 weeks using PCR methods. The first study using knockout moss appeared in 1998 and functionally identified ftsZ as a pivotal gene for the division of an organelle in a eukaryote.
In addition, P. patens is increasingly used in biotechnology. Examples are the identification of moss genes with implications for crop improvement or human health and the safe production of complex biopharmaceuticals in moss bioreactors. By multiple gene knockout Physcomitrella plants were engineered that lack plant-specific post-translational protein glycosylation. These knockout mosses are used to produce complex biopharmaceuticals in a process called molecular farming.
Physcomitrella ecotypes, mutants, and transgenics are stored and made freely available to the scientific community by the International Moss Stock Center (IMSC). The accession numbers given by the IMSC can be used for publications to ensure safe deposit of newly described moss materials.
Like all mosses, the life cycle of Physcomitrella patens is characterized by an alternation of two generations: 1) a haploid gametophyte that produces gametes and 2) a diploid sporophyte where haploid spores are produced.
A spore develops into a filamentous structure called protonema, composed of two types of cells – chloronema with large and numerous chloroplasts and caulonema with very fast growth. Protonema filaments grow exclusively by tip growth of their apical cells and can originate side branches from subapical cells. Some side branch initial cells can differentiate into buds rather than side branches. These buds give rise to gametophores (0.5–5 mm), more complex structures bearing leaf-like structures, rhizoids and the sexual organs: female archegonia and male antheridia. Physcomitrella patens is monoicous, meaning that male and female organs are produced in the same plant. If water is available flagellate sperm cells can swim from the antheridia to an archegonium and fertilize the egg within. The resulting diploid zygote originates a sporophyte composed of a foot, seta and capsule, where thousands of haploid spores are produced by meiosis.
|External identifiers for Physcomitrella patens|
|Encyclopedia of Life||886166|
|Also found in: Wikispecies|
Physcomitrella patens was first described by Johann Hedwig in his 1801 work Species Muscorum Frondosorum, under the name Phascum patens. Physcomitrella is sometimes treated as a synonym of the genus Aphanorrhegma, in which case P. patens is known as Aphanorrhegma patens. The generic name Physcomitrella implies a resemblance to Physcomitrium, which is named for its large calyptra, unlike that of Physcomitrella.
- "!Physcomitrella patens (Hedw.) Bruch & Schimp.". Tropicos. Missouri Botanical Garden. http://www.tropicos.org/name/35108841. Retrieved October 28, 2012.
- Andrew Cuming (2011). "Molecular bryology: mosses in the genomic era" (PDF). Field Bryology 103: 9–13. http://rbg-web2.rbge.org.uk/bbs/Activities/field%20bryology/FB103/FB103%20Cuming.pdf.
- Nick Hodgetts (2010). "Aphanorrhegma patens (Physcomitrella patens), spreading earth-moss". In Ian Atherton, Sam Bosanquet & Mark Lawley (PDF). Mosses and Liverworts of Britain and Ireland: a Field Guide. British Bryological Society. p. 567. ISBN 978-0-9561310-1-0. http://www.bbsfieldguide.org.uk/sites/default/files/pdfs/mosses/Aphanorrhegma_patens.pdf.
- Stefan A. Rensing, Daniel Lang & Andreas D. Zimmer (2009). Comparative genomics. pp. 42–75. doi:10.1111/b.9781405181891.2009.00003.x. http://books.google.co.uk/books?id=IEzoQAH1EdEC&pg=PA42. In: Knight et al. (2009).
- Stefan A. Rensing, Daniel Lang, Andreas D. Zimmer, Astrid Terry, Asaf Salamov, Harris Shapiro, Tomoaki Nishiyama, Pierre-François Perroud, Erika A. Lindquist, Yasuko Kamisugi, Takako Tanahashi, Keiko Sakakibara, Tomomichi Fujita, Kazuko Oishi, Tadasu Shin-I, Yoko Kuroki, Atsushi Toyoda, Yutaka Suzuki, Shin-ichi Hashimoto, Kazuo Yamaguchi, Sumio Sugano, Yuji Kohara, Asao Fujiyama, Aldwin Anterola, Setsuyuki Aoki, Neil Ashton, W. Brad Barbazuk, Elizabeth Barker, Jeffrey L. Bennetzen, Robert Blankenship, Sung Hyun Cho, Susan K. Dutcher, Mark Estelle, Jeffrey A. Fawcett, Heidrun Gundlach, Kousuke Hanada, Alexander Heyl, Karen A. Hicks, Jon Hughes, Martin Lohr, Klaus Mayer, Alexander Melkozernov, Takashi Murata, David R. Nelson, Birgit Pils, Michael Prigge, Bernd Reiss, Tanya Renner, Stephane Rombauts, Paul J. Rushton, Anton Sanderfoot, Gabriele Schween, Shin-Han Shiu, Kurt Stueber, Frederica L. Theodoulou, Hank Tu, Yves Van de Peer, Paul J. Verrier, Elizabeth Waters, Andrew Wood, Lixing Yang, David Cove, Andrew C. Cuming, Mitsuyasu Hasebe, Susan Lucas, Brent D. Mishler, Ralf Reski, Igor V. Grigoriev, Ralph S. Quatrano & Jeffrey L. Boore (2008). "The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants". Science 319 (5859): 64–69. Bibcode 2008Sci...319...64R. doi:10.1126/science.1150646. PMID 18079367. http://www.sciencemag.org/cgi/content/abstract/319/5859/64.
- Didier G. Schaefer & Jean-Pierre Zrÿd (1997). "Efficient gene targeting in the moss Physcomitrella patens". Plant Journal 11 (6): 1195–1206. doi:10.1046/j.1365-313X.1997.11061195.x. PMID 9225463.
- Didier G. Schaefer (2002). "A new moss genetics: targeted mutagenesis in Physcomitrella patens". Annual Review of Plant Biology 53: 477–501. doi:10.1146/annurev.arplant.53.100301.135202. PMID 12221986.
- Annette Hohe, Tanja Egener, JanM. Lucht, Hauke Holtorf, Christina Reinhard, Gabriele Schween & Ralf Reski (2004). "An improved and highly standardised transformation procedure allows efficient production of single and multiple targeted gene-knockouts in a moss, Physcomitrella patens". Current Genetics 44 (6): 339–347. doi:10.1007/s00294-003-0458-4. PMID 14586556.
- René Strepp, Sirkka Scholz, Sven Kruse, Volker Speth & Ralf Reski (1998). "Plant nuclear gene knockout reveals a role in plastid division for the homolog of the bacterial cell division protein ftsZ, an ancestral tubulin". Proceedings of the National Academy of Sciences 95 (8): 4368–4373. doi:10.1073/pnas.95.8.4368. JSTOR 44902. PMC 22495. PMID 9539743. //www.ncbi.nlm.nih.gov/pmc/articles/PMC22495/.
- Ralf Reski & Wolfgang Frank (2005). "Moss (Physcomitrella patens) functional genomics – gene discovery and tool development with implications for crop plants and human health". Briefings in Functional Genomics and Proteomics 4: 48–57. PMID 15975264. http://bfgp.oxfordjournals.org/cgi/content/abstract/4/1/48.
- Eva L. Decker & Ralf Reski (2007). "Moss bioreactors producing improved biopharmaceuticals". Current Opinion in Biotechnology 18 (5): 393–398. doi:10.1016/j.copbio.2007.07.012.
- Anna Koprivova, Christian Stemmer, Friedrich Altmann, Axel Hoffmann, Stanislav Kopriva, Gilbert Gorr, Ralf Reski & Eva L. Decker (2004). "Targeted knockouts of Physcomitrella lacking plant-specific immunogenic N-glycans". Plant Biotechnology Journal 2 (6): 517–523. doi:10.1111/j.1467-7652.2004.00100.x. PMID 17147624.
- Ralf Reski, Merle Faust, Xiao-Hui Wang, Michael Wehe & Wolfgang O. Abel (1994). "Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.". Molecular and General Genetics 244 (4): 352–359. doi:10.1007/BF00286686. PMID 8078460. http://www.springerlink.com/content/h1857x41k341xt67/.
- Bernard Goffinet (2005). "Physcomitrella". Bryophyte Flora of North America, Provisional Publication. Missouri Botanical Garden. http://www.mobot.org/plantscience/bfna//V1/FunaPhyscomitrella.htm. Retrieved October 28, 2012.
- Assaf Mosquna, Aviva Katz, Eva Decker, Stefan Rensing, Ralf Reski & Nir Ohad (2009). "Regulation of stem cell maintenance by the Polycomb protein FIE has been conserved during land plant evolution". Development 136: 2433–2444. doi:10.1242/dev.035048. PMID 19542356.
- Tanja Egener, José Granado, Marie-Christine Guitton, Annette Hohe, Hauke Holtorf, Jan M. Lucht, Stefan A. Rensing, Katja Schlink, Julia Schulte, Gabriele Schween, Susanne Zimmermann, Elke Duwenig, Bodo Rak & Ralf Reski (2002). "High frequency of phenotypic deviations in Physcomitrella patens plants transformed with a gene-disruption library". BMC Plant Biology 2: 6. doi:10.1186/1471-2229-2-6. PMC 117800. PMID 12123528. http://www.biomedcentral.com/1471-2229/2/6.
- Celia Knight, Pierre-François Perroud & David Cove (2009). Preface. pp. xiii–xiv. doi:10.1002/9781444316070.fmatter. http://books.google.co.uk/books?id=IEzoQAH1EdEC&pg=PR13. In: Knight et al. (2009).