The range of distribution for this family is world-wide. It is at its most diverse in North America, with about 297 recognised taxa, but China and Southeast Asia also support very diverse faunas.
Freshwater mussels occupy a wide range of habitats, but most often occupy lotic waters, i.e. flowing water such as rivers, streams and creeks.
Unionoids remove phytoplankton and zooplankton, as well as suspended bacteria, fungal spores, and dissolved organic matter. Despite extensive laboratory studies, it remains uncertain as to which of these filtrates unionoids actually process. In high densities, they have the ability to influence water clarity  but filtration rates are dependent on water temperature, current velocity, and particle size and concentration. In addition, gill morphology can determine particle size filtered, as well as the rate.
The sperm is ejected from the mantle cavity through the male’s excurrent aperture and taken into the female's mantle cavity through the incurrent aperture. Fertilised eggs move from the gonads to the gills (marsupia) where they further ripen and metamorph into glochidia, the first larval stage. Mature glochidia are released by the female and then attach to the gills, fins or skin of a host fish. A cyst is quickly formed around the glochidia, and they stay on the fish for several weeks or months before they fall off as juvenile mussels, which then bury themselves in the sediment.
Some of the species in the Unionidae, commonly known as pocketbook mussels, have evolved a remarkable reproductive strategy. The edge of the female's body that protrudes from the valves of the shell develops into an imitation of a small fish complete with markings and false eyes. This decoy moves in the current and attracts the attention of real fish. Some fish see the decoy as prey, while others see a conspecific, i.e. a member of their own species. Whatever they see, they approach for a closer look and the mussel releases huge numbers of larvae from her gills, dousing the inquisitive fish with her tiny, parasitic young. These glochidial larvae are drawn into the fish's gills, where they attach and trigger a tissue response that forms a small cyst in which the young mussel resides. It feeds by breaking down and digesting the tissue of the fish within the cyst.
Sex is determined by a region located on the mitochondria, the male open reading frame (M-ORF) and female open reading frame (F-ORF). Hermaphroditic mussels lack these regions and contain a female-like open reading frame dubbed hermaphroditic open reading frame (H-ORF). It has been determined that in many mussels, the hermaphroditic state is ancestral and the male sex evolved later. It is also hypothesized that this region of the mitochondria may be responsible for the evolution of Doubly uniparental inheritance (DUI) seen in freshwater mussels.
Fossilization and taphonomic implications
In large enough quantities, unionid shells can have enough of an impact on environmental conditions to affect the ability of organic remains in the local environment to fossilize. For example, in the Dinosaur Park Formation, fossil hadrosaur eggshell is rare because the breakdown of tannins from local coniferous vegetation would have caused the ancient waters to become acidic. Eggshell fragments are present in only two microfossil sites, both of which are dominated by the preserved shells of invertebrate life, including unionids. The slow dissolution of these shells releasing calcium carbonate into the water raised the water's pH high enough to prevent the eggshell fragments from dissolving before they could be fossilized.
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