Acetabularia is a genus of green algae, specifically of the Polyphysaceae family, Typically found in subtropical waters, Acetabularia is a single-celled organism, but gigantic in size and complex in form, making it an excellent model organism for studying cell biology. In form, the mature Acetabularia resembles the round leaves of a nasturtium, is 0.5 to 10 cm tall and has three anatomical parts: a bottom rhizoid that resembles a set of short roots; a long stalk in the middle; and a top umbrella of branches that may fuse into a cap. The single nucleus of Acetabularia is located in the rhizoid, and allows the cell to regenerate completely if its cap is removed. The caps of two Acetabularia may also be exchanged, even from two different species. In addition, if a piece of the stem is removed, with no access to the nucleus in the rhizoid, this isolated stem piece will also grow a new cap.
In the 1930s–1950s Joachim Hämmerling conducted experiments in which he demonstrated Acetabularia's genetic information is contained in the nucleus. This was the first demonstration that genes are encoded by DNA in eukaryotes; earlier studies by Oswald Avery and others had shown that this was true for prokaryotes.
The name, Acetabularia, derives from the Latin word acetabulum, a broad, shallow cup used for dipping bread; the upturned cap of Acetabularia resembles such a cup. For this reason, it is also sometimes called mermaid's wineglass.
Anatomy and life cycle
Acetabularia has three basic parts: its rhizoid, a short set of root-like appendages that contain the nucleus and anchor the cell to fissures in a substrate; its median stalk, which accounts for most of its length; and its apex, where its cap forms. There are usually several whorls of hair-like appendages close to the apex.
Acetabularia are among the largest single-celled organisms, having also a remarkably large nucleus. During sexual reproduction, the nucleus undergoes multiple rounds of mitosis, forming many daughter nuclei all within one nuclear membrane. These nuclei undergo meiosis and are transported to the tips of the branches, the sporangia, where they are released as gametes.
A. mediterranea has a smooth, disc shaped cap, while A. crenulata has a branched, flower-like cap. Each Acetabularia cell is composed of three segments: the "foot" or base which contains the nucleus, the "stalk," and the "cap."
Hämmerling exchanged the caps from two species, A. mediterranea and A. crenulata. The caps of these species are different in form; however, after exchange, the caps gradually changed from their original form to that typical of its base, where the nucleus is located. In another experiment, the addition of a nucleus from another species into a single intact Acetabularia produced a hybrid cap, showing that both nuclei are influencing the form of the cap.
Hammerling's experiment with the single celled green algae, Acetabularia, showed that the nucleus of a cell contains the genetic information that directs cellular development.
Although a single cell, Acetabularia exhibits a remarkably complex shape and has therefore long been a model organism for studying gene expression and morphogenesis. It seems to transport messenger RNA molecules (in an inactive riboprotein form) from the nucleus to its apical tips, where they are translated into proteins. These molecules may be activated by proteolysis of their protein carrier molecules, but this has not been verified as yet.
Internal chemical gradients
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