The species is viviparous (Ref. 5258). Internal fertilization is possible because the anal fin of the male is modified into a copulatory organ. The females carries about 30 alevins and gestation lasts for a period of 24 days (Ref. 6348) to a month (Ref. 30578).
- Seale, A. 1917 The mosquito fish, Gambusia affinis (Baird and Girard), in the Philippine Islands. Philipp. J. Sci. 12(3):177-189. (Ref. 6348)
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