Syngnathoides biaculeatus has a wide geographic range. It has been recorded in surveys and taxonomic overviews from the Red Sea and the African east coast to Knysna, South Africa (Dawson 1985, Dawson 1986). It is also found in the Indo-Pacific from the east coast of India, throughout the South China Sea and has been recorded as far northward as northern Japan. It has been found in three states and one territory in Australia: Western Australia, the Northern Territory, Queensland, and New South Wales (Paxton et al. 1989). It has been recorded near the islands of Micronesia and Samoa (Randall et al. 1997).

Australian Marine Protected Areas in Which Has Been Recorded (Pogonoski et al. 2002): In Australia, S. biaculeatus is found in the following marine protected areas: Ashmore Reef National Nature Reserve, off northern Western Australia, Cartier Island Marine Protected Area, Great Barrier Reef Marine Park, Queensland, and CoburgMarinePark, in the Northern Territory (Pogonoski et al. 2002).

Museum Records Worldwide: in Australia, there are 106 specimens (standard length 100-288 mm), collected from a depth range of 0 to 5 m, ranging in geographical distribution from the Timor Sea, Northern Territory south-eastwards to Batemans Bay (35°44’S), New South Wales on the east coast of Australia, and from Ashmore Reef (12°13’S) southwards to Geraldton (28°46’S) on the west coast of Australia. Outside Australia there are specimens from the Andaman Islands, India, Malay Archipelago, Guam, Papua New Guinea and the Solomon Islands. Specimens were collected between circa 1879 and 1998 (Pogonoski et al. 2002).

Other museum records are available from FishBase and the Ocean Biogeographic Information System (OBIS) and include specimens collected from Suva Harbor in Fiji, Singapore, Kenya, and Yemen (Froese and Pauly 2007, Ocean Biogeographic Information System 2007). Specimens listed in Fishbase/OBIS have collection dates from 1828 to 2004.