Not much is known about the preferred habitat of Monodelphis kunsi. Based on the topography of the sites from which the specimens were collected, it appears that M. kunsi occupy a wide range of terrestrial habitats. Locations from which the specimens were collected are characterized by a wide range of elevations, from 200 m to 1500 m. One specimen of the pygmy short-tailed opossum was collected in an area with thick brush, many rocks and fallen wood, and ground covered by occasional small forbs and ferns. The ground was steeply sloped (30°) and there was about 10 to 12 cm layer of mulch and litter. In contrast, another specimen was collected in a banana field about 30 m from a river. On this flat terrace with very moist soil and about 2 cm of leaf litter; the trees (7 to 8 m tall) and shrubs combined, provided 30 to 60 % cover. While these two areas represent somewhat moderately disturbed ecosystems, pygmy short-tailed opossums have also been collected in quite pristine habitats.
Range elevation: 200 to 1500 m.
Habitat Regions: tropical ; terrestrial
Terrestrial Biomes: rainforest
Other Habitat Features: agricultural