Comments: Based on patterns of genetic and morphological variation, Pauly et al. (2007) concluded that Ambystoma cingulatum should be split into two species, A. bishopi west of the Apalachicola-Flint rivers and A. cingulatum east of those rivers.
Based on mtDNA evidence, A. cingulatum (sensu stricto) inhabiting the eastern Gulf Coastal Plain and the Atlantic Coastal Plain in northern Florida are distinct lineages; the Suwannee River and a large distributional gap separate them (Pauly et al. 2007). Further data are needed to determine whether these two lineages warrant recognition as different species (Pauly et al. 2007).