The head is as wide as it is long, and rounded when viewed dorsally (Savage 2002). The snout is rounded in profile (Savage 2002). Eyes are large, with gray-gold irises and black reticula, and the pupils are horizontal (Savage 2002). The dorsal skin is granular, with widely scattered bumps (Savage 2002). There is a fleshy fold on the forearm, along the posterior lower edge (Savage 2002). Finger and toe disks are present and truncate (Savage 2002). The outer fingers are webbed, and the toes are moderately webbed (Savage 2002). Males have white nuptial pads on the thumbs (Savage 2002).
Their appearance mostly resembles Fleishmann's Glassfrog and the Powdered Glassfrog. Fleishmann's Glassfrog is lighter in color than the Yellow-flecked Glassfrog and has white bones, while the Powdered Glassfrog has larger spots (Guyer and Donnelly, 2005).
The karyotype is 2N=20 (Duellman 1967).
A Spanish-language species account can be found at the website of Instituto Nacional de Biodiversidad (INBio).