The Bartholomea annulata are members in a number of symbiotic relationships. They host zooxanthellae, Symbiodinium sp. dinflagellate algae that grow within the cells of the anemone aiding in biological functions and providing color.
They are also part of an association with a number of Crustacea species. Many small crustaceans such as the Periclimenes yucatanicus and the Alpheus armatus receive protection within the tentacles of the anemone and also may perform cleaning functions for the anemone. ()