The breeding season in Florida coincides with the wet season, persisting from May through October (Behler 1979). Beginning around March and lasting through the breeding season, the distinctive grating/scraping mating croak of calling males is commonly detected (Duellman and Schwartz 1958, GSMFC).Males mature at around 40 mm length and the much larger females mature at 50 mm or larger. Mating occurs in ponds and along lake margins, in drainage ditches and other ephemeral water bodies, and in pools, cisterns, and other manmade structures. During mating, females will lay an egg mass that is externally fertilized by males. The fertilized egg mass floats in a sheet at the water's surface until hatching (Behler 1979, Ashton and Ashton 1988). The size of the egg mass varies based on the size of the female; numbers ranging from 130 to 3,000 eggs have been reported. Large females can produce more than 15,000 eggs during the course of a breeding season (Johnson 2007).
- Allen E.R., and W.T. Neill. 1953. The treefrog Hyla septentrionalis in Florida. Copeia 1953:127-128.
- Ashton R.E., and P.S. Ashton. 1988. Handbook Of Reptiles And Amphibians of Florida. Part Three The Amphibians. Windward Publishing, Inc., Lakeville MN. 191 p.
- Banks R.C., McDiarmid R.W, Gardner A.L., and W.C. Starnes. 2004. Checklist of vertebrates of the United States, the U.S. Territories,and Canada. U.S. Dept of Interior. 79 p.
- Behler J.L. 1979. The Audubon Society Field Guide to North American Retiles and Amphibians. Alfred A. Knopf, Inc. New York. 743 p.
- Carmichael P., and W. Williams. 1991. Florida's Fabulous Reptiles and Amphibians. World Publications, Tampa, FL. 120 p.
- Conant R., and J.T. Collins. 1991. Reptiles And Amphibians. Eastern/Central North America. Houghton Mifflin Company, Boston. 450 p.
- Duellman W.E., and A. Schwartz. 1958. Amphibians and reptiles of southern Florida. Bulletin of the Florida Museum 3:181-324.
- Duellman W.E., and R.I. Crombie. 1970. Pages 92.1-92.4 in: Reimer W.J. (Ed.). Catalogue Of American Amphibians And Reptiles. American Society Of Ichthyologists And Herpetologists.
- Johnson S.A. 2006. The Cuban Treefrog (Osteopilus septentrionalis) in Florida. UF/IFAS document is WEC218. Available online.
- King W. 1960. New populations of West Indian reptiles and amphibians in southeastern Florida. Quarterly Journal of the Florida Academy Science 23:71-73.
- Mattison C. 1987. Frogs and Toads of the World. Facts On File Publications, New York. 191 p.Myers S. 1977. Geographic distribution: Osteopilus septentrionalis. Herpetology Review 8:38.
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