The native range of O. septentrionalis includes Cuba, the Isla de Pinos, the Bahamas, and the Cayman Islands (Duellman and Crombie 1970).Cuban treefrogs now occur throughout peninsular Florida, with breeding populations found as far north as Jacksonville on the east coast and as far north as Cedar Key on the Gulf Coast. Individuals (but not breeding pairs) have been reported from the Florida panhandle and also from coastal Georgia and South Carolina (Johnson 2007). A population introduced to Hawaii has reportedly been extirpated (Banks et al. 2004). Osteopilus septentrionalis is established in all six counties of the India River Lagoon watershed.
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