Anolis longitibialis shares its habitat with the Common Ameiva, Ameiva chrysolaema. While the anole perches within a meter and a half of the ground, A. chrysolaema is primarily a ground-dwelling lizard. Anolis longitibialis is also found with the Barahona Curlytail Lizard, Leiocephalus barahonensis, on piles of limestone rocks in Jaragua National Park, in the Barahona region of the southwestern Dominican Republic. In Jaragua, but not on Isla Beata, A. longitibialis occurs sympatrically, though rarely syntopically, with A. cybotes, a closely related trunk-ground anole. In appearance these species are relatively easy to distinguish. However, they share some similarities in perching habits, despite early accounts reporting strong microhabitat differences between the two species. Anolis cybotes, which is more characteristically found on tree trunks, may sometimes be observed perching on rocks, and A. longitibialis may sometimes be observed on tree trunks and other vegetation. On Isla Beata the only other anole species that A. longitibialis occurs with is A. brevirostris, a small anole that is a member of the “trunk” ecomorph, but the latter is primarily restricted to tree trunks and branches and is not commonly found on the rocks that A. longitibialis tends to prefer. These species occur syntopically, especially on the sea grape (Coccoloba) trees lining the beach and in the dry forest.